Discovery and identification of three bioactive compounds affecting endothelial function in\nGinkgo biloba Extract (GBE) based on chromatogram-bioactivity correlation analysis. Three portions\nwere separated from GBE via D101 macroporous resin and then re-combined to prepare nine GBE\nsamples. 21 compounds in GBE samples were identified through UFLC-DAD-Q-TOF-MS/MS.\nCorrelation analysis between compounds differences and endothelin-1 (ET-1) in vivo in nine GBE\nsamples was conducted. The analysis results indicated that three bioactive compounds had\nclose relevance to ET-1: Kaempferol-3-O-�±-L-glucoside, 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-\n�²-D-glucosyl}-�±-rhamnosyl} Quercetin isomers, and 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-\n�²-D-glucosyl}-�±-rhamnosyl} Kaempferide. The discovery of bioactive compounds could provide\nreferences for the quality control and novel pharmaceuticals development of GRE. The present work\nproposes a feasible chromatogram-bioactivity correlation based approach to discover the compounds\nand define their bioactivities for the complex multi-component systems.
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